Developing and validation of High Performance Liquid Chromatography method for determination of carboplatin-loaded Metal-Organic Framework materials

Abstract

Introduction: Carboplatin is widely used in cancer treatments despite several adverse effects. Metal-organic frameworks nanoparticles (MOF) were used to load carboplatin, which could improve selectivity to cancerous tissues and control the release profile of this compound. This study aimed to develop and validate a sensitive, simple, cost-effective RP-HPLC method for quantifying carboplatin in MOF.

Methods: This study was developed and optimal different chromatographic conditions for quantification of carboplatin-loaded MOF materials. The chosen HPLC procedure was validated throughout ICH guidelines.

Results: The chromatographic separation was achieved on an RP-C18 column (250 x 4.6 mm, 5 µm) by isocartic elution with a mobile phase consisting of methanol: potassium chloride 0.9% in a 1:1 ratio. The flow rate is 1.0 mL/min. PDA detector was used at a wavelength of 254 nm. The validation process has achieved the following criteria: system suitability, specificity, linearity, accuracy, precision, range of determination, and robustness. The method showed linearity in the 0.1-1.5 mg/mL range with R² = 0.9998. The accuracy and %RSD were 98.03-101.37% (mean 99.98%), and 1.08, respectively.

Conclusion: This HPLC method can be used to determine the carboplatin loading and desorption efficiency of MOF materials, including ZIF-8, Zr-UiO-66, and Hf-UiO-66. The method provides a reliable analytical tool for complex MOF compositions, ensuring safety and efficacy of novel drug delivery systems while accelerating future development in this field.

 

Keywords: Carboplatin, HPLC, quantitative evaluation, MOF materials, drug delivery systems.