Science & Technology Development Journal - Health Sciences

The effect of 1% povidone iodine and 0,75% boric acid on chronic periodontitis treatment

2020-12-13T22:36:41+00:00

Objective: To evaluate the effect of 0,75% boric acid or 1% povidone iodine irrigation an adjunct to scaling and root planning (SRP) in treatment of chronic periodontitis patients.

Methods: A singlemasked, randomized, clinical trial with 40 chronic periodontitis patients was selected at Facultly of Odonto-Stomatology, University of Medicine and Pharmacy, Ho Chi Minh city. They were randomly divided into two treatment groups depending on the irrigation solution: Group 1, SRP plus 1% PVP-I irrigation Group 2, SRP plus 0,75% boric acid irrigation. Clinical measurements including bleeding on probing (BOP), probing depth (PD) and clinical attachment level (CAL) were obtained; and quantitative analyses of Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg) were performed using real-time polymerase chain reaction (PCR) procedures at baseline (T0), 4 weeks (T4), 8 weeks (T8) and 12 weeks (T12) after periodontitis treatment.

Results: The PD and CAL parameters in shadow, moderate and severe pockets; and amount of Aa, Pg showed statistically significant reduction at T4, T8, and T12 compared to T0 in both groups. Between the two groups, whole mouth BOP reduction was significantly bigger in group 2 compared to group 1 at T4, T8 and T12. The PD and CAL reductions were significantly greater in group 2 compared to group 1 at T4, and T8 and T12 for shadow, moderate and deep pockets; and at T4 for deep pockets. The reductions in amount of Aa, Pg were greater in group 2 compare to group 1 at T4, T8 and T12, however, statistical differences were not found.

Conclusion: The results of this study suggest that 0,75% boric acid might be more effective than 1% povidone-iodine in treatment for patients with chronic periodontitis.

Development and validation of a HPLC method for simultaneous quantitative determination of adapalene and benzoyl peroxide in gel products

2020-12-27T13:59:08+00:00

Adapalene (AP) and Benzoyl peroxide (BO) have been used to treat acnes very efficient for many years, especially in gel products. But nowaday, that gel products still don't have in-house standard. And furthermore, the most difficult thing to build in-house standard is to assay simultaneously. The aim of this research is to build a method to assay two of AP and BO simultaneously by HPLC method. Subjects: AP (from India) and BO (from China). Solvents get the quality standard to use in liquid chromatography. Sample: gel product (AP 0.1% and BO 2.5%) prepared at Laboratory of School of Medicine, National University of HCMC. Chromatographic system: HPLC Shimadzu system with column C18 RP (250 x 4.6 mm; 5 mm), injection volumn 20 mL, detector at 270 nm, flow rate 1 mL/min, temperature 30oC, mobile phase with acetonitril - tetrahydrofuran - acid formic 0.1% in water 42 : 32 : 26. Validations: system suitability, specificity, linearity, accuracy, precision adapt to ICH. Results: The HPLC method for simultaneous quantitative determination of adapalene and benzoyl peroxide in gel products was built successfully. The method was achieved all validations term adapt to ICH: system suitability, specificity, linearity, accuracy, precision. Furthermore, that method is used to assay the concentration of two API (AP and BO) in the product (prepared at Laboratory of School of Medicine, National University of HCMC) with result: the concentration of AP is 101.38 0.87% and that of BO is 95.56 0.04 %, get the standard of USP 41.

Prevalence of arrhythmias in elderly inpatients at department of cardiology of Thong Nhat Hospital

2020-12-27T14:02:06+00:00

Background: Arrhythmia is becoming an important condition of the elderly with high prevalence and frequency with increasing age.

Objectives: To definite prevalence of arrhythmias in elderly inpatients treated at the Department of Cardiology of Thong Nhat Hospital - Ho Chi Minh City from January 2016 to December 2016 and the relationship between arrhythmias and some associated diseases.

Methods: Retrospective study incorporating a descriptive cross-sectional study, performing a full read-back of the routine ECG on 1024 patients treated at the Department of Cardiology of Thong Nhat Hospital.

Results: The rate of arrhythmias in the age 60 years and more is 51%. Arrhythmias include atrial arrhythmias (30.6%), conduction disorders (22.3%) and ventricular arrhythmias (6.6%). Atrial fibrillation accounts for 10.7%.

Conclusions: Arrhythmias are frequent in the elderly and equal between men and wonmen. Atrial fibrillation is the most common arrhythmia in the elderly and gradually increases in men after age of 60. There is no difference in the prevalence of arrhythmias among the elderly and younger people.

In vitro cytotoxic activity against human hepatoma HepG2 cells, antioxidant activity, total phenol and flavonoid content of methanol extracts from four plants in Bay Nui, An Giang Province

2020-12-27T14:07:13+00:00

Liver cancer has become a common cancer in Viet Nam and in the world. The in vitro study of the cytotoxicity on hepatocellular carcinoma cell line HepG2 is important for testing for active substances that are actually useful in treating liver cancer. In this study, based on literature and folk herbal knowledge in the use of Vietnamese medicinal herbs to treat cancer and liver diseases, we have evaluated cytotoxicity against HepG2, antioxidant capicity, total flavonoid and phenol content of methanol extracts from Luvunga scandens leaves (RLD1), Hyptis suaveolens (L.) roots (RLD9), Solanum torvum (Sw.) leaves (RLD10) and Zingiber zerumbet (L.) rhizomes (RLD7R). As a result, at 100 mg/L concentration of the extracts, RLD1 and RLD9 demonstrated the highest cytotoxicity with cell survival rate less than 27%. While RLD1 sample showed weak antioxidant capacity (IC50 = 426.275 0.005 mg/L), RLD9 possessed the stronger antioxidant activity (IC50 = 24.320 0.031 mg/L) as well as the highest total phenol content (97.441.46 mg GAE/g extract). Although RLD7R sample did not show any cytotoxic activity against HepG2 cells, it exhibited a strong antioxidant potential (IC50 = 21.326 0.083 mg/L), associated with the highest total flavonoid content (63.13 14.30 mg QE/g extract). This study has demonstrated that Luvunga scandens leaves and Hyptis suaveolens roots have potential in liver cancer treatment.