Latest Articles

  1. Case Reports

    A novel KCNQ2 gene mutation in a patient with self-limitted non-familial neonatal epilepsy: a case report

    NGUYEN T., H., NGUYEN Q., M., LE K., V., LE M., T., LE A., N., DO H., HUYNH H., DO T. H.
    This study reported a rare case of self-limited non-familial neonatal epilepsy with a novel KCNQ2 gene mutation. It should be cautious that some patients with KCNQ2-self-limited non-familial neonatal epilepsy may present some features resembling epileptic encephalopathy at onset/early stage. The complex genotype–phenotype correlation of KCNQ2-related epilepsies and the genetic counselling involved with the case are also discussed in this case report.
    Published: 2021-02-2194-101
  2. Original research

    High expression of DIMT1 correlates with poor prognosis and high risk of bone metastasis in breast cancer patients

    Tran H., Vo T., Nguyen M. N.
    DIMT1 is a potential predictor in gaining insights into the heterogeneous landscape of bone metastatic. Moreover, by integrating into the molecular subtypes, DIMT1 can further subclassify patients into two risk groups, thereby enabling more informed therapeutic decisions and improved prognostics for breast cancer. Together, these findings indicate the general utility of DIMT1 as a biomarker in the age of personalized and precision medicine.
    Published: 2021-02-0852-63
  3. Original research

    In vitro cytotoxic activity against human hepatoma HepG2 cells, antioxidant activity, total phenol and flavonoid content of methanol extracts from four plants in Bay Nui, An Giang Province

    Tri L., Nghia C., Nhi N., Ngan H., Nguyen H.
    Liver cancer has become a common cancer in Viet Nam and in the world. The in vitro study of the cytotoxicity on hepatocellular carcinoma cell line HepG2 is important for testing for active substances that are actually useful in treating liver cancer. In this study, based on literature and folk herbal knowledge in the use of Vietnamese medicinal herbs to treat cancer and liver diseases, we have evaluated cytotoxicity against HepG2, antioxidant capicity, total flavonoid and phenol content of methanol extracts from Luvunga scandens leaves (RLD1), Hyptis suaveolens (L.) roots (RLD9), Solanum torvum (Sw.) leaves (RLD10) and Zingiber zerumbet (L.) rhizomes (RLD7R). As a result, at 100 mg/L concentration of the extracts, RLD1 and RLD9 demonstrated the highest cytotoxicity with cell survival rate less than 27%. While RLD1 sample showed weak antioxidant capacity (IC50 = 426.275 0.005 mg/L), RLD9 possessed the stronger antioxidant activity (IC50 = 24.320 0.031 mg/L) as well as the highest total phenol content (97.441.46 mg GAE/g extract). Although RLD7R sample did not show any cytotoxic activity against HepG2 cells, it exhibited a strong antioxidant potential (IC50 = 21.326 0.083 mg/L), associated with the highest total flavonoid content (63.13 14.30 mg QE/g extract). This study has demonstrated that Luvunga scandens leaves and Hyptis suaveolens roots have potential in liver cancer treatment.
    Published: 2020-12-3186-93
  4. Original research

    Development and validation of a HPLC method for simultaneous quantitative determination of adapalene and benzoyl peroxide in gel products

    Nguyen V.,-H., Pham T.,-Q., Nguyen T.,-B.,-A., Bui T.,-P., Thai T.,-T., Pham N.,-Q., Ly L.,-D., Le M.-T.
    Adapalene (AP) and Benzoyl peroxide (BO) have been used to treat acnes very efficient for many years, especially in gel products. But nowaday, that gel products still don't have in-house standard. And furthermore, the most difficult thing to build in-house standard is to assay simultaneously. The aim of this research is to build a method to assay two of AP and BO simultaneously by HPLC method. Subjects: AP (from India) and BO (from China). Solvents get the quality standard to use in liquid chromatography. Sample: gel product (AP 0.1% and BO 2.5%) prepared at Laboratory of School of Medicine, National University of HCMC. Chromatographic system: HPLC Shimadzu system with column C18 RP (250 x 4.6 mm; 5 mm), injection volumn 20 mL, detector at 270 nm, flow rate 1 mL/min, temperature 30oC, mobile phase with acetonitril - tetrahydrofuran - acid formic 0.1% in water 42 : 32 : 26. Validations: system suitability, specificity, linearity, accuracy, precision adapt to ICH. Results: The HPLC method for simultaneous quantitative determination of adapalene and benzoyl peroxide in gel products was built successfully. The method was achieved all validations term adapt to ICH: system suitability, specificity, linearity, accuracy, precision. Furthermore, that method is used to assay the concentration of two API (AP and BO) in the product (prepared at Laboratory of School of Medicine, National University of HCMC) with result: the concentration of AP is 101.38 0.87% and that of BO is 95.56 0.04 %, get the standard of USP 41.
    Published: 2020-12-3164-74
  5. Original research

    The effect of 1% povidone iodine and 0,75% boric acid on chronic periodontitis treatment

    Pham T., Tran Q.ên
    To evaluate the effect of 0,75% boric acid or 1% povidone iodine irrigation an adjunct to scaling and root planning (SRP) in treatment of chronic periodontitis patients.
    Published: 2020-12-3175-85
  6. Original research

    Prevalence of arrhythmias in elderly inpatients at department of cardiology of Thong Nhat Hospital

    Ho D., Trần Bác., Mai Hữu Đỗ P.,úc Lê P.,ươn., Trần T., Nguyen C.
    Arrhythmia is becoming an important condition of the elderly with high prevalence and frequency with increasing age.
    Published: 2020-12-2744-51
  7. Original research

    Impact of Nucleic Acid Testing (NAT) on screening blood donors at a tertiary center in Vietnam

    To K., Lâm T.,ị N.,ọc T.,âm Nguyễn P.,ươn., A., Hồ Tấn T., Lò T.,ị T.,ảo N.,ên Cai T.,ị T., N.,ân Phù C.í Dũn.
    To ensure safe blood transfusion, since June 2015, Nucleic Acid Testing (NAT) technique has been applied in blood screening process in some areas in Vietnam. The objective of our project is to examine the effectiveness of this technique, to provide specific, updated evidence, as the fundamental for promoting the application of NAT technology in screening blood donors. The study describes a series of cases, performed on 412,705 blood donated samples meeting inclusion criteria at Blood Transfusion Hematology Hospital from January 1, 2017 to January 1, 2019. All collected blood samples were screened for HBV, HCV, and HIV by serology technique, then samples with negative results will continue to be screened by NAT technique. Test results and donor information will be stored on the the hospital’s database. As a result, thanks to NAT screening, our study found 369 more positive blood samples with viral genetic material that serology did not detect, of which: 352 samples reacted with HBV-DNA (0.0871%), 10 samples responded to HCV-RNA (0.0025%) and 6 samples responded to HIV-RNA (0.0017%). Therefore, the application of NAT to the blood screening process is necessary to improve the safety of blood transfusion. Regarding the characteristics of positive cases with infectious agents, we recorded a higher rate of positive for HBV, HIV than men, even when tested by serology or by NAT technique (statistically significant, P <0.05); The positive rate for these 3 agents was higher for first-time blood donors than for repeat blood donors, statistically significant with serological screening (P <, 001) and not statistically significant when screening NAT (P> 0.05). In conclusion, The application of NAT technology in blood screening is essential and effective, improving blood transfusion safety.
    Published: 2020-11-1735-43
  8. Original research

    An optimized method for detection of MECP2 point mutations in Vietnamese children with rett syndrome

    LÊ VÂN LÊ DƯƠN., ĐỖ HẰN., HUỲNH K.,ỀU NGUYỄN LÊ H.,ẾU BÙI D., NGUYỄN VŨ NGUYỄN DŨN., HUYNH H.
    Rett syndrome is a rare disease but affecting severe brain dysfunction. The rate of the syndrome is estimated to be 1/10,000–1/15,000 female births. This disorder is knowledge caused by de novo mutations in the MECP2 gene. More than 80% of patients with Rett syndrome have a point mutations in MECP2. The study correlation genotype-phenotype open to therapeutic opportunities for patients. In Vietnam, to validate the diagnosis, the doctors are mainly clinical diagnostic standards, not include genetics testing. Therefore, optimize the producer to determine MECP2 variations is necessary that provides evidence for clinical diagnosis, supporting the earlystage diagnosis of pediatric patients.
    Published: 2020-11-1027-34
  9. Original research

    Pancytopenia in children at hematology department, children hospital 1: clinico-haematological profiles and bone marrow examination – a case series

    Duong M., Thanh An D., T., My L.
    To determine causes of pancytopenia in children via bone marrow aspiration at Hematology Department, Children hospital 1; clinical characteristics, time to diagnostic; peripheral blood smear and bone marrow examination.
    Published: 2020-10-2318-26
  10. Original research

    Histopathological features of chronic periapical lesions

    Pham D., L., Vo T., Nguyen K. C.
    Chronic periapical inflammation, the sequela of necrotic pulp, is one of the most common diseases in dental practice. The diagnosis of these lesions is based on clinical presentation, radiological features and histopathological examination. The aim of this study was to evaluate the clinico-pathological features of chronic inflammatory periapical lesions. 92 periapical lesions were collected at HCMC Odonto-Stomatology Hospital and HCMC National Odonto-Stomatology Hospital at HCM from June 2019 to January 2020. Male to female ratio was 1:1.6, the age range was 10-70 with average age was 35.5 years old. In terms of clinical features, 30.4% of patients were asymptomatic, 28.3% had percussion sensitivity, 27.2% had swelling, 12% had cortical expansion, 18.5% had intraoral fistula and 3.3% had tooth mobility. The most frequently- involved locations were the anteria maxilla and posterior mandible. Histopathological results confirmed 49 radicular cysts and 43 periapical granulomas. In most cases of radicular cyst (47 cases, 96%), the cyst was lined with nonkeratinized stratified squamous epithelium, with two cases containing mucous cells, 3 cases were observed with cholesterol clefts and 3 cases with Hyaline bodies. In 43 cases of periapical granulomas, the lesions included chronic inflammatory cells and 3 cases with cholesterol clefts. The collagen fibers in fibrous stroma of radicular cysts appeared to be thicker and more linearly arranged while they seemed to be thinner in scattered pattern in periapical granulomas. There was no significant difference between the prevalence of radicular cysts (53.3%) and periapical granulomas (46.7%), no case of chronic periapical abscess was recorded. The ratio of radicular cysts (67.3%) was significantly higher than periapical granulomas (41.9%) at the posterior areas (p<0.05).
    Published: 2020-09-069-17

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