TY - JOUR AU - Tran Thao AU - Luong Ngan AU - Nguyen Van AU - Pham Thuy PY - 2021/08/05 Y2 - 2024/03/29 TI - Isolation and storage of Aggregatibacter actinomicetemcomitans from the subgingival plaque of patients with periodontitis JF - Science and Technology Development Journal: Health Sciences JA - STDJHS VL - 2 IS - 2 SE - Original research DO - https://doi.org/10.32508/stdjhs.v2i2.467 UR - http://stdjhs.scienceandtechnology.com.vn/index.php/stdjhs/article/view/467 AB - Objective: To isolate and identification Aggregatibacter actinomicetemcomitans from clinical subgingival plaques of periodontitis patients. Methods: Selective media was prepared by adding 200 ug/mL of vancomycin and 10 U/mL of bacitracin, 5% sheep blood to Wilkins Chalgren Anaerobic agar. Subgingival samples collected from patients with moderate to severe periodontitis aged 18- 35 were used to recover Aa. The colony was then identified by the MALDI-TOF method and PCR analysis. Results: The Aa was successfully cultured and stored from a clinical sample of the 12th 25 years-old patient who had moderate aggressive periodontitis. Conclusion: Aa is a major putative oral pathogen and concerned with other systemic diseases. This pure bacterial culture will be helpful for other experimental studies in dental and medical areas. ER -